Hot Water Extraction of Antibacterials from Eggs

February 2, 2009

Researchers from Universita ?La Sapienza? (Rome, Italy) used a matrix solid-phase dispersion technique with hot water as the extracting solvent to extract seven quinolone antibacterial agents from egg samples.

Researchers from Universita “La Sapienza” (Rome, Italy) used a matrix solid-phase dispersion technique with hot water as the extracting solvent to extract seven quinolone antibacterial agents from egg samples. They used liquid chromatography with tandem mass spectrometry to analyze the extracted analytes. The researchers deposited the egg sample containing the analytes on crystobalite sand and packed it into an extraction cell. Acidified water was pumped through the cell, which was maintained at 100 °C. They validated the method in terms of ruggedness, specificity, linearity, within-laboratory reproducibility, and detection capability, and afterward conducted a depletion study of enrofloxacin and ciprofloxacin in eggs.