Impact of Gradient Delay Volume on Impurity Separation



Webinar Date/Time: Monday, June 24, 2024 at 10am EDT | 3pm BST | 4pm CEST

An Improved Chlorhexidine Digluconate Impurity Separation Demonstrating Batch Reproducibility.

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Event Overview:

Chlorhexidine is a disinfectant and antiseptic widely used for skin disinfection prior to surgery, cleaning wounds, and treating oral infections. The current European Pharmacopeia chlorhexidine digluconate monograph has proved problematic. In this webinar, the proposed draft monograph is evaluated, considering multiple batches of HPLC columns, as well as the impact of system dwell volume on the overall performance and robustness of the monograph method.

Key Learning Objectives:

  • Why is it required to achieve system sustainability criteria in LC analysis
  • Why is batch to batch reproducibility test important in method validation
  • Why and when must gradient delay volume be considered
  • Understand the importance of choosing the "right" column and "correct" gradient elution for separating a great number of impurities

Who Should Attend:

  • Laboratory Managers
  • Analysts
  • HPLC Operators


Amra Perva
University of Maribor, Faculty of Chemistry and Chemical Engineering

Amra Perva is employed as a research fellow at the faculty of chemistry and chemical engineering at the University of Maribor (Slovenia). She received a master’s degree with a thesis on the isolation of active ingredients from chile pepper and green tea by high pressure extractions. She moved to the analytical department in 2007 after obtaining her PhD in chemical engineering with a thesis on phase equilibria for designing a high pressure micronisation process for vanillins and cocoa butter. As the project lead in a GMP certified contract research organization (Group for Separation Analysis), she has more than a decade of experience working with pharmaceutical companies in the field of LC and GC method development, validation, stability and release testing for drug substances and drug products. She is responsible for delivering results obtained in compliance with monographs for assay and related substances.

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