LC for Determination of Sulfamonomethoxine, Sulfadimethoxine, and their N4-Acetyl Metabolites in Eggs

August 7, 2007

In an article recently published in Analytica Chimica Acta, researchers describe the simultaneous determination of sulfamonomethoxine, sulfadimethoxine, and their N4-acetyl metabolites in eggs using restricted-access media high performance liquid chromatography (RAM-HPLC) with a photo-diode array detector.

In an article recently published in Analytica Chimica Acta, researchers describe the simultaneous determination of sulfamonomethoxine, sulfadimethoxine, and their N4-acetyl metabolites in eggs using restricted-access media high performance liquid chromatography (RAM-HPLC) with a photo-diode array detector. According to the article, the target compounds are extracted by a handheld ultrasonic homogenizer with saturated ammonium sulfate solution followed by centrifugation.

The separation is performed by a Hisep shielded hydrophobic phase column, isocratic elution with 0.3% acetic acid solution–ethanol. Average recoveries from samples spiked at 0.1–1.0 ppm for each drug were >91% with relative standard deviations within 4%. No hazardous-chemicals were used in all the processes.

To view, please visit:

http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T6R-4JGJGVW-2&_user=10&_coverDate=12%2F31%2F2007&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=f85e54fc20e6371579183cce888973b7