LC for Determination of Sulfamonomethoxine, Sulfadimethoxine, and their N4-Acetyl Metabolites in Eggs

In an article recently published in Analytica Chimica Acta, researchers describe the simultaneous determination of sulfamonomethoxine, sulfadimethoxine, and their N4-acetyl metabolites in eggs using restricted-access media high performance liquid chromatography (RAM-HPLC) with a photo-diode array detector. According to the article, the target compounds are extracted by a handheld ultrasonic homogenizer with saturated ammonium sulfate solution followed by centrifugation.

The separation is performed by a Hisep shielded hydrophobic phase column, isocratic elution with 0.3% acetic acid solution–ethanol. Average recoveries from samples spiked at 0.1–1.0 ppm for each drug were >91% with relative standard deviations within 4%. No hazardous-chemicals were used in all the processes.

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