Market Profile: UHPLC
Ultrahigh-performance liquid chromatography (UHPLC) systems are designed to take full advantage of smaller particles (sub 2 μm), which yield higher resolution and increased sensitivity. Because of increased back pressure, UHPLC systems employ high-performance pumps that deliver 15 000 psi (1,000 bar) or more of pressure. Some systems solve the back-pressure issue by elevating temperatures using a column oven, which lower the viscosity of the mobile phase, allowing it to move faster through the column. UHPLC systems have been shown to provide analysis times of less than 1 min and up to 40 times faster than conventional HPLC systems.
Cloruson and Related Substances Studied Using Original Ion-Pair UHPLC Method
March 19th 2024In a recent study out of Boehringer Ingelheim Animal Health USA Inc. in North Brunswick, New Jersey, scientists investigated cloruson and its related substances using an original ion-paired reversed phase ultraperformance liquid chromatography (IP-UHPLC) method.
UHPLC–MS Method Used to Detect Purine Metabolites and Study Severe Biological Disorders
December 5th 2023A recent study published in the Journal of Separation Science reveals how ultrahigh-performance liquid chromatography tandem mass spectrometry (UHPLC–MS/MS) could be used to detect and quantify purine metabolites.