The Journal of Chemical Physics reports that researchers are aiming to selectively ionize molecules for mass spectrometry.
The Journal of Chemical Physics reports that researchers are aiming to selectively ionize molecules for mass spectrometry. The goal is to make protein sequencing a bit faster by combining the nice parts of mass spectrometry (speed) and enzyme digestion (specificity). In current systems, the protein molecule is shattered by a huge blast of energy and the mass of the charged fragments is measured. By understanding how proteins shatter, you can figure out a range of different sequences, which must then be refined by other techniques. This is very fast, but difficult to interpret. Since every chemical reaction involves breaking a bond and (usually) ionizing the fragments, the researchers decided to use pulse shaping to only break a specific bond in the protein backbone. The work holds some promise in speeding up the analysis of huge biomolecules, although still in the early stages.
Transferring Methods to Compact and Portable HPLC
February 14th 2024The current trend in laboratory equipment design is the miniaturization of laboratory instruments. Smaller-scale HPLC instruments offer benefits that cannot be matched by analytical-scale equipment, especially in the areas of portability, reduced fluid volumes, and reduced operating costs. Yet, the miniaturization of laboratory equipment has brought with it a unique set of challenges, including transferring methods to compact LC. Capillary LC expands the use of LC to applications not currently done using conventional LC in a wide array of application areas, including pharmaceutical, food and beverage, petrochemical, environmental, and oil and gas. Greg Ward, Axcend’s CEO wrote, “Customers want an HPLC system with a small footprint, low flow rates and green chemistry.” Join his podcast where he shares method transfer in these application areas.