Membrane-Assisted Cleanup of Biological Extracts
Researchers from the UFZ-Helmholtz Centre for Environmental Research (Leipzig, Germany) used an accelerated membrane-assisted cleanup technique for the analysis of organic compounds in lipid-rich biological samples.
Researchers from the UFZ-Helmholtz Centre for Environmental Research (Leipzig, Germany) used an accelerated membrane-assisted cleanup technique for the analysis of organic compounds in lipid-rich biological samples. A Dionex (Sunnyvale, California) ASE device was used to automate the technique. Semipermeable low-density polyethylene membranes enabled recoveries greater than 70% for the organic compounds from the lipid-rich matrix. The technique provided a higher matrix removal capacity than gel permeation chromatography.
Study Explores Thin-Film Extraction of Biogenic Amines via HPLC-MS/MS
March 27th 2025Scientists from Tabriz University and the University of Tabriz explored cellulose acetate-UiO-66-COOH as an affordable coating sorbent for thin film extraction of biogenic amines from cheese and alcohol-free beverages using HPLC-MS/MS.
Multi-Step Preparative LC–MS Workflow for Peptide Purification
March 21st 2025This article introduces a multi-step preparative purification workflow for synthetic peptides using liquid chromatography–mass spectrometry (LC–MS). The process involves optimizing separation conditions, scaling-up, fractionating, and confirming purity and recovery, using a single LC–MS system. High purity and recovery rates for synthetic peptides such as parathormone (PTH) are achieved. The method allows efficient purification and accurate confirmation of peptide synthesis and is suitable for handling complex preparative purification tasks.
