Chemical contaminants that are released into the marine environment may be ingested (absorbed) by fish and shellfish and thus become introduced into the human food chain.
Chemical contaminants that are released into the marine environment may be ingested (absorbed) by fish and shellfish and thus become introduced into the human food chain. Lipophillic chemicals such as OCPs and PCBs can bioaccumulate in the fatty tissues of marine fish and shellfish. The longer an organism is exposed to a contaminated environment, the higher the likely levels of contaminants
The Clean Seas Environmental Monitoring Program (CSEMP) is an initiative designed to monitor the levels of chemical contamination in the United Kingdom's coastal and estuarine areas. The major drivers for this program are:
Agilent Technologies has partnered with a leading European Analytical Laboratory to develop a sample preparation method based on a modified QuEChERS extraction along with a GC/MS/MS method for the determination of selected OCPs, PAHs, and PCBs in marine shellfish (Mussel) tissue. The GC/MS/MS method provides reproducible and sensitive determination of OCPs, PCBs, and PAHs that employs large-volume (solvent vent) injection using a Multimode inlet (MMI) and post-column, post-run backflush in order to remove high-boiling matrix components that would otherwise remain in the column between analyses and subsequently cause degradation of chromatographic performance and contamination of the mass spectrometer ion source.
Figure 1. TIC MRM chromatogram of a calibration standard mixture of OCPs, PAHs and PCBs*
The analytical method meets the detection limit requirements of 0.1 µg/Kg for OCPs and PCBs, and 0.5–1.0 µg/Kg for PAHs.
Figure 2. MRM chromatograms for (I) incurred a-HCH and (II) incurred g-HCH in mussel sample, concentrations 0.06 and 0.30 Âµg/Kg, respectively. Peaks (III) and (IV) are traces of incurred b-HCH and d-HCH, respectively
Figure 3. MRM chromatograms for (I) incurred fluoranthene and (II) incurred pyrene in mussel sample, Concentrations 8.64 and 5.83 Âµg/Kg, respectively
* Full analytical details are available in Agilent Technologies publication 5990-7714EN.
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