N-Glycan Analysis of Biotherapeutics: Does N-Glycan Sample Prep Matter?

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Webinar Date/Time: Thu, May 18, 2023 11:00 AM EDT

Compare and contrast the reproducibility, throughput, and ease of use of leading N-glycan sample preparation kits. Fluorescence signal and MS ionization efficiency of released and labeled N-glycans from the same amount of glycoprotein will be highlighted, to aid researchers in advancing their biotherapeutics and drug development process.

Register Free: https://www.chromatographyonline.com/lcgc_w/n-glycan-analysis

Event Overview:

Glycosylation can be a critical quality attribute (CQA) or product quality attribute (PQA) of biotherapeutic proteins, as the structure of N-linked glycans can influence protein function. Analysis of released N-glycans labeled with a dye that facilitates hydrophilic interaction liquid chromatography (HILIC) with detection by fluorescence (FLD) and/or mass spectrometry (MS) is a commonly used approach. The sample preparation step of this workflow determines data quality, consistency, and ease of use.
This webinar will:

  • Present Agilent N-glycan sample preparation and HILIC/FLD/MS analysis workflows
  • Discuss a side-by-side comparison of Agilent’s Gly-X InstantPC sample preparation option for this workflow with another option on the market (Waters RapiFluorMS), covering FLD and MS signal, reproducibility, and ease of use
  • Show data on the preparation and analysis of released N-glycans from different biotherapeutic glycoproteins, such as rituximab (monoclonal antibody), etanercept and abatacept (Fc fusion proteins), as well as a control protein (NISTmAb).

Key Learning Objectives:

  • Factors that influence selection of a sample preparation workflows for N-glycans on biotherapeutics
  • HILIC LC/FLD/MS approaches for N-glycan analysis, including examples from biotherapeutics and control proteins

Featured Speaker:

Dr. Aled Jones
Marketing Manager,
Agilent Technologies

Aled Jones is the marketing manager for bioconsumables at Agilent, covering a portfolio that includes glycan sample preparation and standards. He received his BSc and PhD degrees in biochemistry from Cardiff University.

Register Free: https://www.chromatographyonline.com/lcgc_w/n-glycan-analysis