Native HIC-MS of ADCs for DAR Species Determination and Identification (Aug 2025)

By using a streamlined native hydrophobic interaction chromatography-mass spectrometry (HIC-MS) method, MS-compatible ammonium tartrate salts can enhance the separation and detection of antibody-drug conjugate (ADC) drug-to-antibody ratio (DAR) species. This advanced approach overcomes historical coupling difficulties and supports rapid, high-resolution DAR profiling and real-time monitoring for improved ADC characterization and manufacturing control.