The first presentation in the session will be given by Cong Wu of the Department of Biochemistry at the University of Illinois (Urbana, Illinois) and The Institute of Genomic Biology (Urbana, Illinois), and is titled ?An Efficient and Restricted Protease for Middle Down Proteomics.? Wu will discuss a highly efficient and restricted proteolytic approach to produce large peptides that empowers robust and routine middle down proteomics.
The first presentation in the session will be given by Cong Wu of the Department of Biochemistry at the University of Illinois (Urbana, Illinois) and The Institute of Genomic Biology (Urbana, Illinois), and is titled “An Efficient and Restricted Protease for Middle Down Proteomics.” Wu will discuss a highly efficient and restricted proteolytic approach to produce large peptides that empowers robust and routine middle down proteomics.
The next talk, to be delivered by Glenn A. Harris of Vanderbilt University (Nashville, Tennessee), is titled “Localized In-Situ Micro Hydrogel-Mediated Protein Digestion and Extraction For Imaging Mass Spectrometry” and will discuss hydrogel facilitated, simultaneous on-tissue digestion and extraction protocol for MALDI-IMS and LC-MS-MS identification on a single biological tissue section.
Michael J. Roth of the University of Texas Southwestern Medical Center (Dallas, Texas) will present the next talk, “Surface Preparation Instead of Sample Preparation: Accurate Quantitation Without Internal Standards or Separations.” Roth’s presentation will discuss high-throughput, reproducible label-free analysis of protein species by MALDI-MS immunoassays as an alternative to MRM and colorimetric immunoassays.
The fourth presentation in the session, “Converting Proteomic Based Discovery into a Validation Assay for Cardiac Ischemic Biomarkers,” will be delivered by Robert J. Cotter of Johns Hopkins School of Medicine (Baltimore, Maryland). The presentation will discuss how automating sample preparation for an SRM-MS assay using the Perfinity Workstation made their SRM-MS assay viable as an ED diagnostic.
The penultimate presentation will be given by Jordan Aerts of the University of Illinois at Urbana-Champaign (Champaign, Illinois) and is titled “Subcellular Analysis of Individual Neurons with Capillary Electrophoresis Electrospray Mass Spectrometry.” The talk will describe methods for subcellular sampling and unambiguous determination of the source of ion signals by subcellular CE-MS.
Finally, Shuwen Sun of the University of Michigan (Ann Arbor, Michigan) will present “High Throughput Screening Using Electrospray Mass Spectrometry by Droplets.” This presentation will demonstrate the concept of using a droplet-ESI-MS system by performing the screening Cathepsin B inhibitors.
The Next Frontier for Mass Spectrometry: Maximizing Ion Utilization
January 20th 2025In this podcast, Daniel DeBord, CTO of MOBILion Systems, describes a new high resolution mass spectrometry approach that promises to increase speed and sensitivity in omics applications. MOBILion recently introduced the PAMAF mode of operation, which stands for parallel accumulation with mobility aligned fragmentation. It substantially increases the fraction of ions used for mass spectrometry analysis by replacing the functionality of the quadrupole with high resolution ion mobility. Listen to learn more about this exciting new development.
Using Chromatography to Explore Fluorescent Bioactive Compounds in the Coelomic Fluid of Earthworms
February 6th 2025A study aiming to identify fluorescent bioactive compounds in the coelomic fluid of earthworms, as well as to investigate their structural and functional characteristics for possible use in biomedical applications, utilized a variety of chromatographic techniques.
The Complexity of Oligonucleotide Separations
January 9th 2025Peter Pellegrinelli, Applications Specialist at Advanced Materials Technology (AMT) explains the complexity of oligonucleotide separations due to the unique chemical properties of these molecules. Issues such as varying length, sequence complexity, and hydrophilic-hydrophobic characteristics make efficient separations difficult. Separation scientists are addressing these challenges by modifying mobile phase compositions, using varying ion-pairing reagents, and exploring alternative separation modes like HILIC and ion-exchange chromatography. Due to these complexities, AMT has introduced the HALO® OLIGO column, which offers high-resolution, fast separations through its innovative Fused-Core® technology and high pH stability. Alongside explaining the new column, Peter looks to the future of these separations and what is next to come.