Overcoming Challenges in MS Analysis Through Improvements in Sample Preparation

The first presentation in the session will be given by Cong Wu of the Department of Biochemistry at the University of Illinois (Urbana, Illinois) and The Institute of Genomic Biology (Urbana, Illinois), and is titled “An Efficient and Restricted Protease for Middle Down Proteomics.” Wu will discuss a highly efficient and restricted proteolytic approach to produce large peptides that empowers robust and routine middle down proteomics.

The next talk, to be delivered by Glenn A. Harris of Vanderbilt University (Nashville, Tennessee), is titled “Localized In-Situ Micro Hydrogel-Mediated Protein Digestion and Extraction For Imaging Mass Spectrometry” and will discuss hydrogel facilitated, simultaneous on-tissue digestion and extraction protocol for MALDI-IMS and LC-MS-MS identification on a single biological tissue section.

Michael J. Roth of the University of Texas Southwestern Medical Center (Dallas, Texas) will present the next talk, “Surface Preparation Instead of Sample Preparation: Accurate Quantitation Without Internal Standards or Separations.” Roth’s presentation will discuss high-throughput, reproducible label-free analysis of protein species by MALDI-MS immunoassays as an alternative to MRM and colorimetric immunoassays.

The fourth presentation in the session, “Converting Proteomic Based Discovery into a Validation Assay for Cardiac Ischemic Biomarkers,” will be delivered by Robert J. Cotter of Johns Hopkins School of Medicine (Baltimore, Maryland). The presentation will discuss how automating sample preparation for an SRM-MS assay using the Perfinity Workstation made their SRM-MS assay viable as an ED diagnostic.

The penultimate presentation will be given by Jordan Aerts of the University of Illinois at Urbana-Champaign (Champaign, Illinois) and is titled “Subcellular Analysis of Individual Neurons with Capillary Electrophoresis Electrospray Mass Spectrometry.” The talk will describe methods for subcellular sampling and unambiguous determination of the source of ion signals by subcellular CE-MS.

Finally, Shuwen Sun of the University of Michigan (Ann Arbor, Michigan) will present “High Throughput Screening Using Electrospray Mass Spectrometry by Droplets.” This presentation will demonstrate the concept of using a droplet-ESI-MS system by performing the screening Cathepsin B inhibitors.