Optimizing sample preparation is critical for generating high-quality, reproducible analytical data, particularly when working with complex matrices. Yet, it often demands significant time and effort. Traditional methods, including solid phase extraction (SPE), supported liquid extraction (SLE), filtration, and nucleic acid (NA) extraction, rely on column- or plate-based protocols and can face limitations in throughput, reproducibility, and the handling of difficult samples. Conventional approaches such as centrifugation and vacuum manifolds introduce further challenges, from added manual intervention and inconsistent flow to difficulty processing viscous samples. Positive pressure instrumentation uses controlled gas flow to push liquids through columns or plates, maintaining constant pressure across all positions to improve analyte recovery, reduce matrix effects, and support automation. This whitepaper examines how positive pressure compares to conventional methods and how Tecan's Resolvex platforms apply the technology across manual, semi-automated, and fully automated workflows.
In this fourth part of " Conversations with Michael Dong," a series on fundamentals of HPLC in pharmaceutical analysis, LCGC International’s "Perspectives in HPLC" column editor explains why buffers are most often used in Mobile Phase A in high-pressure liquid chromatography of pharmaceuticals.
Quantitative non-targeted screening with predicted response factors is not for regulation, but can rank environmental chemicals by concentration and toxicity.
Gas chromatography–mass spectrometry (GC–MS) reveals a four-stage polycyclic aromatic hydrocarbons (PAH) breakdown in oil sludge co-composted with manure.
