
- The Column-10-22-2010
- Volume 6
- Issue 19
Power plant
Following the accident at Chernobyl Nuclear Power Plant in 1986, the surrounding area remains substantially contaminated with radioactive isotopes. A study into the adaptations of the surrounding plant life has suggested that the plant proteomes remain largely unchanged by the radio contamination.
Following the accident at Chernobyl Nuclear Power Plant in 1986, the surrounding area remains substantially contaminated with radioactive isotopes. A study into the adaptations of the surrounding plant life has suggested that the plant proteomes remain largely unchanged by the radio contamination.1
The explosion at Chernobyl released large quantities of radionuclides into the environment. Since then the situation has stabilized into one of persistent low-dose chronic exposure, which the local flora and fauna have adapted to. Previously, genomic and proteomic approaches have been applied to study these adaptations but this study employed high-throughput quantitative methods for protein identification. Flax seeds were grown on contaminated ground before a total protein fraction was isolated from mature seeds and analysed using 2-dimensional electrophoresis combined with tandem-mass spectrometry. Surprisingly, radiation exposure had relatively little effect on the protein levels in the plants, with only about 5% of the proteins altered, researchers noted. UHPLC with MSE was applied to these 35 proteins and allowed researchers to identify 28 of them. Among them were proteins involved in cell signalling or chemical communication, and the team suggests this may be what allows the plants to shrug off radioactivity.
1. K. Klubicová, Environ. Sci. Technol., 44, 6940–6946 (2010).
Articles in this issue
about 15 years ago
Changing namesabout 15 years ago
Multiple reaction monitoring atlasabout 15 years ago
Purifying proteinsabout 15 years ago
Summer Reflections (Part 2)about 15 years ago
Q&A: Sourcing It Outabout 15 years ago
Market Profile: UHPLCNewsletter
Join the global community of analytical scientists who trust LCGC for insights on the latest techniques, trends, and expert solutions in chromatography.





