Quantitative Analysis by MS in Drug Discovery and Development

This session includes presentations of quantitative studies performed using mass spectrometry techniques ranging from ultratrace analysis of small molecules in complex samples, to bioanalysis of microsamples, to LC–MS-MS analysis at low flow rates, and MS applications in clinical toxicology.

The first presentation in the session will be given by Bennett N. Walker of George Washington University (Washington, D.C.) and is titled “Nanophotonic Ion Production for Ultra-Sensitive Direct Mass Spectrometry of Complex Samples.” Bennett will present a study of efficient ion production in laser desorption ionization from silicon nanopost arrays and small molecule quantitation in complex mixtures.

The next talk, to be delivered by Nicholas Manicke of Quantion Technologies (West Lafayette, Indiana), is titled “Analysis of Biological Samples by Paper Spray-MS: Toward Point of Care Mass Spectrometry” and will discuss the rapid and direct quantitation of drugs in blood and the development of a method for collecting samples at clinical sites.

Jack Henion of Advion Bioanalytical Labs, Inc. (Ithaca, New York) will present the next talk, “Development of Quantitative Nanoscale Techniques for Ultra Trace Analysis of Biological Microsamples.” Henion’s presentation will discuss the use of a nanoscale liquid chromatography–selected reaction monitoring mass spectrometry method for analyzing samples in microdosing studies.

The fourth presentation in the session, “Investigation of Lower LC Flow Rates and Smaller ID Columns in Quantitative Bioanalysis by LC-MS/MS,” will be delivered by Richard King of PharmaCadence Analytical Services, LLC (Hatfield, Pennsylvania). The presentation will discuss a quantitative comparison of electrospray ionization sensitivity, linear dynamic range, and lower limit of quantitation as a function of liquid chromatography flow rate.

The penultimate presentation will be given by Guowen Liu of Bristol-Myers Squibb (Princeton, New Jersey) and is titled “LC-MS/MS Bioanalysis of Monoclonal Antibody Drugs in Drug Development: Practical Considerations in Assay Development and Validation.” The talk will discuss practical LC–MS solutions to high-throughput protein quantitation in regulated bioanalysis.

Finally, Russell Grant of Labcorp. (Burlington, North Carolina) will present “Design and Utility of High Throughput Open-Access Liquid Chromatography Tandem Mass Spectrometry in Quantitative Clinical Toxicology.” This presentation will discuss open-access design and application of a targeted quantitation system for clinically relevant drug compounds.