Researchers Improve Sensitivity of Large Volume Urine Injections with HPLC-MS

Researchers at the Chinese Academy of Sciences and Tsingua University have developed an automated on-line HPLC-MS method allowing injection of a large volume of urine for the improvement of sensitivity using estrogens as analytes.

Researchers at the Chinese Academy of Sciences and Tsingua University have developed an automated on-line HPLC-MS method allowing injection of a large volume of urine for the improvement of sensitivity using estrogens as analytes. The injection volume, reporetedly, increased ten times compared with conventional on-line LC-MS methods for biofluid analysis. Moreover, no obvious increase of column pressure was observed after 300 injections. The method was validated by spiking urine. Linearity was determined by sample which was in the range of 1-500 ng/mL. The process efficiency ranged from 70.2 to 106% with RSDs less than 15% with the exception of a few analytes.