The Secrets to Successful Gradient Separations

Liquid chromatography separations using gradient elution have the potential to be faster, give better detection limits, and have more flexibility than separations using isocratic elution. However, problems can occur with gradients. Some of these problems are inherent to the technique but can be avoided or accommodated with a little care. Others result from a poor understanding of gradient elution. In this new e-book, John Dolan, renowned LC expert and the longtime author of the "LC Troubleshooting" column in LCGC, presents the secrets to achieving success with gradient separations.