Separation and Scale-Up of an Achiral Compound Utilizing the RegisCell® CSP

June 1, 2009

The Application Notebook

The Application Notebook, The Application Notebook-06-01-2009, Volume 0, Issue 0

Although supercritical fluid chromatography (SFC) is generally regarded as a technique to separate enantiomers, it has also been proven in many laboratories as a fast approach for the purification of impurities in achiral samples.

Eric E. Pullen, Andrew G. Geyer, and Vinay M. Edwin, Regis Technologies, Inc.

Although supercritical fluid chromatography (SFC) is generally regarded as a technique to separate enantiomers, it has also been proven in many laboratories as a fast approach for the purification of impurities in achiral samples.

Figure 1

A proprietary active pharmaceutical ingredient (API) was examined by both RP-HPLC and SFC methodology for the elimination of a ~0.8% impurity. The compound of interest and the impurity have a M.W. > 350 daltons and differ structurally by only an acetamide versus a formamide group.

The best RP-HPLC method found for the separation was isocratic with a high percentage of an R-OH solvent with H20 on a C18 column.

Figure 2

A method utilizing 35% MeOH in CO2 on a RegisCell® column was developed for SFC. Figure 1 illustrates the analytical separation and subsequent loading calculations.

Experimentally Determined Production Rates

Single Column:

25 cm X 4.6 mm – 190 mg/h

25 cm X 21.1 mm – 4.6 gm/h

Serially Coupled Columns:

Two - 25 cm X 4.6 mm – Not performed

Two - 25 cm X 21.1 mm – 16 gm/h

Conclusions

As shown, the ability to separate the desired compound from the main impurity would be extremely time consuming and costly by RP-HPLC on a large scale. However, by using SFC the gains in efficiency are obvious and significant with this sample. Extrapolation of the experimentally derived data to two 25 cm X 50 mm columns would result in a production rate of over 90 grams/h.

Regis Technologies, Inc.

8210 Austin Ave, Morton Grove, IL 60053

tel. (847)583-7667, fax (847)967-1214

Website: www.registech.com