Solid-Phase Microextraction Used in Mycotoxin Analysis
A research group from the University of Waterloo (Waterloo, Ontario, Canada) has used solid-phase microextraction (SPME) coupled with liquid chromatography-tandem mass spectrometry (LC-MS-MS) to determine the mycotoxin ochratoxin A in human urine.
A research group from the University of Waterloo (Waterloo, Ontario, Canada) has used solid-phase microextraction (SPME) coupled with liquid chromatography-tandem mass spectrometry (LC-MS-MS) to determine the mycotoxin ochratoxin A in human urine. A multifiber SPME device was used with microplates for increased sample throughput. The method reportedly required minimal sample pretreatment to adjust sample pH. Ochratoxin A in urine is a good marker for human exposure to the mycotoxin, and the method is less invasive than blood analysis.
Study Explores Thin-Film Extraction of Biogenic Amines via HPLC-MS/MS
March 27th 2025Scientists from Tabriz University and the University of Tabriz explored cellulose acetate-UiO-66-COOH as an affordable coating sorbent for thin film extraction of biogenic amines from cheese and alcohol-free beverages using HPLC-MS/MS.
Multi-Step Preparative LC–MS Workflow for Peptide Purification
March 21st 2025This article introduces a multi-step preparative purification workflow for synthetic peptides using liquid chromatography–mass spectrometry (LC–MS). The process involves optimizing separation conditions, scaling-up, fractionating, and confirming purity and recovery, using a single LC–MS system. High purity and recovery rates for synthetic peptides such as parathormone (PTH) are achieved. The method allows efficient purification and accurate confirmation of peptide synthesis and is suitable for handling complex preparative purification tasks.
