SP-FT for Rapid Analysis Using Conventional HPLC
IEC SP-FT 4A
- Separated five standard proteins at 3.0 mL/min
- Maintained the same separationat 1.5 mL/min flow rate
- Analysis can be completed in under 1 min
SP-FT for Rapid Analysis using conventional HPLC
Sample: 5 µL
1. Ovalbumin 0.8 mg/mL
2. Trypsinogen 1.6 mg/mL
3. Ribonuclease A 1.6 mg/mL
4. Cytochrome c 0.8 mg/mL
5. Lysozyme 0.4 mg/mL
IEC SP-FT 4A
- Nonporous type column
- Cation exchange chromatography
- Ultra rapid and high resolution
- Analyzed Hemoglobin A, F, S, and C
Shodex™/Showa Denko America, Inc.
420 Lexington Avenue, Suite 2335A, New York, NY 10170
tel. (212) 370-0033, fax (212) 370-4566
Website: www.shodexHPLC.com
SEC-MALS of Antibody Therapeutics—A Robust Method for In-Depth Sample Characterization
June 1st 2022Monoclonal antibodies (mAbs) are effective therapeutics for cancers, auto-immune diseases, viral infections, and other diseases. Recent developments in antibody therapeutics aim to add more specific binding regions (bi- and multi-specificity) to increase their effectiveness and/or to downsize the molecule to the specific binding regions (for example, scFv or Fab fragment) to achieve better penetration of the tissue. As the molecule gets more complex, the possible high and low molecular weight (H/LMW) impurities become more complex, too. In order to accurately analyze the various species, more advanced detection than ultraviolet (UV) is required to characterize a mAb sample.
