The Analysis of Biological Systems is Addressed at The Chromatography Forum of Delaware Valley 2023 Annual Symposium

The Chromatography Forum of Delaware Valley (CFDV) Annual Symposium, being held tomorrow, April 20, 2023, in Claymont, Delaware, will feature an oral afternoon session from 1:00–2:45 pm. This session is titled, “Analysis of Biological Systems,” and will be chaired by Monica Joshi of West Chester University. Like the morning session, it will feature three talks.

Mitosis - cell division of bacteria. 3D rendered illustration. | Image Credit: © vchalup - stock.adobe.com

Pierluigi Delmonte of the U.S. Food and Drug Adminstration (FDA) will open the session with a talk titled, “Challenges in Trans-fat Analysis and Optimization of Parameters for Accurate Quantitation.” Delmonte will discuss the use of gas chromatography with flame ionization detection (GC-FID) to measure trans fatty acids (t-FAs) and other fatty acids in food, and will also address the limitations of the technique. In his work, a single comprehensive method was developed that permits the quantification of t-FAs in food. He will discuss how variability can affect the separations of fatty acids when using highly polar capillary columns, and how a new tuning procedure was developed that allows for reproducible separations, which helps contribute to more accurate quantifications of t-FAs.

Daniel Steyer of GSK will continue the conversation with a talk titled, “Application of Liquid Chromatography–Mass Spectrometry and Droplet Microfluidics for Monitoring Pancreatic Islet Secretions.” Steyer and his team have developed complementary methods for analyzing islet secretions from microfluidic experimentation. He will describe how liquid chromatography–mass spectrometry (LC–MS) afforded broad monitoring of secreted analytes, and how their work applying chromatographic techniques to biomedical analysis can improve the treatment of patients—in this case, those afflicted with diabetes mellitus.

Constantinos Pistos of West Chester University will conclude the session with a talk titled, “Green Analytical Toxicology: What is the Future in Forensic Toxicology?” In his talk, Pistos will review alternative techniques for preparing forensic samples for analysis. Techniques that will be discussed include solid-phase microextraction (SPME), which is a miniaturized derivation of SPE, hollow fiber liquid-phase microextraction (HF-LPME), dispersive liquid-liquid microextraction (DLLME), and switchable hydrophilicity solvent-based homogenous liquid–liquid microextraction (SHS-HLLME). Pistos will explain why these techniques are “greener” than techniques commonly used for forensic sample analysis, including SPE and LLE.

All three talks are designed to reinforce the theme of this year’s annual symposium. For more information about the CFDV and their events, please click here: https://www.cfdv.org/