Tips for Achieving Consistent Performance and Long Life of UHPLC Columns when Using Water-Acetonitrile Gradients with Water Soluble Buffers

Reversed-phase ultra high performance liquid chromatography (RP-UHPLC) columns are the most widely used UHPLC columns in analytical separations. As with other reversed-phase columns, analytes are separated in RP-UHPLC columns due to their differences in the degree of hydrophobic interaction with the stationary phase. In our bacterial metabolomic studies we used a C18 UHPLC column with MS detection. Due to its smaller particle size packing, the UHPLC column provided a very high efficiency separation. The shorter column length and smaller internal diameter helped to achieve high speed separations and reduced mobile phase solvent consumption, thus decreasing cost and environmental impact.

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