Tips & Tricks: GPC/SEC Result Uncertainty - How Reliable Are Results?
GPC/SEC results and their validity are crucial in many applications, for example, as part of a QC/QA test for a product release, the registration and accreditation of polymeric products or formulations with regulatory agencies such as the FDA, REACH and many more. Obviously, the accuracy and precision of GPC/SEC results is an important issue in such a context.
GPC/SEC results and their validity are crucial in many applications, for example, as part of a QC/QA test for a product release, the registration and accreditation of polymeric products or formulations with regulatory agencies such as the FDA, REACH and many more. Obviously, the accuracy and precision of GPC/SEC results is an important issue in such a context.
Influence of Concentration in Conventional GPC/SEC and Advanced Detection GPC/SEC
March 21st 2025Sample concentration is a parameter that can influence the quality of gel permeation chromatography/size-exclusion chromatography (GPC/SEC) separations and the obtained results. Understanding this influence can help to support the development of reliable GPC/SEC methods.
Multi-Step Preparative LC–MS Workflow for Peptide Purification
March 21st 2025This article introduces a multi-step preparative purification workflow for synthetic peptides using liquid chromatography–mass spectrometry (LC–MS). The process involves optimizing separation conditions, scaling-up, fractionating, and confirming purity and recovery, using a single LC–MS system. High purity and recovery rates for synthetic peptides such as parathormone (PTH) are achieved. The method allows efficient purification and accurate confirmation of peptide synthesis and is suitable for handling complex preparative purification tasks.
