Trouble-Free UHPLC-Like Performance Using 2 mm I.D. Monolithic Silica Columns

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Tuesday, June 22, 2021 at 10am EDT | 3pm BST | 4pm CEST Leverage UHPLC-like performance using monolithic 2 mm-I.D. columns. Increase lab efficiency with trouble-free, fast, and efficient HPLC of challenging and matrix-rich samples.

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Event Overview:

The performance of HPLC columns has improved dramatically in terms of separation power by a reduction in particle size. However, not all samples benefit equally from this development: Clean samples with a relatively simple matrix are ideally suited for sub-2-µm UHPLC columns. But for samples with a more difficult and complex matrix, the benefits are often accompanied by significant limitations, which results in increased need for sample preparation. Monolithic 2-mm I.D. columns overcome these limitations due to their robust bi-modal silica structure. In this webcast, we will discuss how monolithic 2-mm columns provide:

  • Cost and time efficient HPLC analysis of matrix-rich samples with simplified sample preparation
  • Rapid, UHPLC-like separations at significant lower column backpressure
  • Excellent separation power for small and large molecule analysis

Key Learning Objectives:

  • Understand the analytical challenges in fast and ultra-fast U/HPLC separations and how to overcome them
  • Learn how to leverage lab efficiency by simplifying the HPLC workflow using monolithic silica columns
  • Discover the UHPLC-like performance of monolithic 2 mm I.D. columns for small molecule separation and for the fast separation of biomolecules

Who Should Attend:

  • HPLC and UHPLC users for small- and large-molecule separation in all industries
  • Researchers in universities performing LC-MS and UHPLC analysis
  • Method developers in the pharmaceutical industry and biopharma


Petra Lewits
Global Product Manager for Analytical Chromatography, HPLC columns
The Life Science Business of Merck KGaA, Darmstadt, Germany

Petra Lewits has worked with chromatography for more than 30 years. She started her career in separation science in 1983 using several chromatographic methods, including HPLC, TLC, GC and solid-phase extraction (SPE), for food control in a governmental institution lab. In 1986 she joined Merck KGaA, Darmstadt, Germany in the pharmaceutical R&D and QC department where she developed HPLC methods for R&D and QC of pharmaceutical APIs and formulations as well as for diet convenience foods. From 1991 on she supported customers in different positions with technical and application-related inquires in Europe as a sales specialist for HPLC instruments, also providing technical support for chromatography and complaint management. Since 2002 she has been a Global Product Manager for Analytical Chromatography (HPLC). In 2014 she began focusing on thin-layer chromatography (TLC). Since April 2018 Petra has had global responsibility for analytical HPLC columns for the life science business of Merck KGaA, Darmstadt, Germany.

Benjamin Peters
Lab Head Instrumental Analytics R&D
The Life Science Business of Merck KGaA, Darmstadt, Germany

Benjamin Peters has been a lab manager at Merck KGaA in Darmstadt, Germany, since 2015. His lab develops products for instrumental analytics, with a focus on titration, high-purity solvents, and analytical chromatography. Before taking over responsibility as a lab manager, Benjamin worked in different laboratories, focusing on process and analytical chromatography in quality control as well as in research and development. The main focus of his work was based on the synthesis of silica materials and the design of surface bonding for various chromatographic applications as well as the physico-chemical analysis of these materials. He joined the life science business of Merck KGaA in Darmstadt, Germany in 2006 as an apprentice for a chemical lab technician.

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