|Articles|September 1, 1999

LCGC North America

  • September 1999
  • Volume 17
  • Issue 9
  • Pages: 836–840

Validation Viewpoint: The International Conference on Harmonization

In past "Validation Viewpoint" columns, we briefly discussed the International Conference on Harmonization (ICH), but we would like to explain the organization in more detail. This month's column describes the ICH and provides an update of ICH projects and guidelines.

LCGC 17(9), 836–840 (1999).

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Chromatography Detects Toxins in Tea Samples
High-performance liquid chromatography-tandem mass spectroscopy (HPLC–MS/MS) chromatography reveals pyrrolizidine alkaloids (PA)/pyrrolizidine alkaloid N-oxides (PANO) toxin risks in 63 tea samples.
Figure 2. Split injection used to measure triplicate test of MTBE, hexane, o-xylene, and 1-methylnaphthalene comparing different solvents (methanol, methylene chloride) and column insertion distances starting with 0.5 mm, 5.0 mm, 10.0 mm, 15 mm, and 20.0 mm. Methanol had the best performance at the 5.0 mm insertion distance and methylene chloride looked slightly better at the 0.5 mm. We would still recommend not going below the 5.0 mm manufacturer recommended insertion distance.
Revisiting insertion distance for methanol and methylene chloride in GC analysis and confirming that insertion depth strongly affects response and reproducibility.