Fast HPLC Method Development Using Interfaced Photodiode-Array and Ion-Trap MS Detection

The authors developed an analytical method for the fast analysis of vitamins in a complex matrix using in-line and complementary high performance liquid chromatography with photodiode-array and mass spectrometry detection techniques. The method development was significantly faster when the authors used both the UV-vis spectral scanning capability of a photodiode-array detector and the mass spectral scanning capability of an ion-trap mass spectrometer.