Andrew Coffey | Authors

Articles

Making the Most of Size‑Exclusion Bioseparations Using Sub‑2‑µm Column Technology

Size-exclusion chromatography (SEC) is a mainstay in the biopharmaceutical industry, serving as a gold standard analytical tool for the characterization of therapeutic proteins in development and manufacturing settings. Contemporary SEC separations can be performed using columns packed with sub-2-μm particles, and these platforms offer the highest efficiencies available for the separation of monoclonal antibody monomer species from low- and high-molecular-weight product-related impurities. Compared to other chromatographic modes used to characterize proteins, SEC is unique in that analytes are not retained by the stationary phase. As a result, special care is required to achieve in practice the chromatographic efficiency that is expected in theory. In this article, we describe the fundamental aspects of achieving high performance using sub-2-μm SEC columns. In addition, we discuss trends in the biopharmaceutical industry, including challenges that can be addressed using modern size-exclusion technologies.

Fast, High-Resolution Size Exclusion Chromatography of Aggregates in Biotherapeutics

This application note describes techniques to increase sample throughput without compromising the accuracy of analysis during the monitoring of aggregate formation during biotherapeutic development

Fast, High-Resolution Size Exclusion Chromatography of Aggregates in Biotherapeutics

This application note describes techniques to increase sample throughput without compromising the accuracy of analysis during the monitoring of aggregate formation during biotherapeutic development