Connor Flannery

Hydrophilic interaction chromatography–mass spectrometry (HILIC-MS) offers a flexible and efficient alternative to ion-pairing reversed-phase liquid chromatography (IP-RPLC) for oligonucleotide analysis, with column selectivity and mobile phase pH being key factors in optimizing retention and detection.

A hybrid HILIC–ion exchange column was used for the analysis of ultrashort-chain per- and polyfluoroalkyl substances (PFAS) compounds in environmental waters. This direct injection LC–MS method enables simultaneous measurement of ultrashort- chain, alternative, and legacy PFAS in potable and non-potable waters.

The 47th International Symposium on High Performance Liquid Phase Separations and Related Techniques (HPLC 2018), chaired by Norman Dovichi, was held from 29 July to 2 August in Washington, D.C., USA. This instalment of “Column Watch” covers some of the highlights observed at the symposium. In addition, trends and perspectives on future developments in HPLC noted from the conference are presented.

The 47th International Symposium on High Performance Liquid Phase Separations and Related Techniques (HPLC 2018), chaired by Norman Dovichi, was held from 29 July to 2 August in Washington, D.C., USA. This instalment of “Column Watch” covers some of the highlights observed at the symposium. In addition, trends and perspectives on future developments in HPLC noted from the conference are presented.

Topics that stood out at HPLC 2018 include advances in large-molecule separations, multidimensional separations, 3D printing, chiral separations, and HILIC. Also, many speakers addressed separation fundamentals, including ways to increase speed, generate higher throughput, and add selectivity.