Antec

HDX-MS with online EC reduction enables successful epitope mapping of mAbs to challenging S-S bonded protein targets, e.g., VEGF. The biophysical characterization of VHH-IR4, a BBB-crossing single-domain antibody (sdAb) is described for the first time and was only possible using EC reduction.

High-Performance Anion Exchange Chromatography in combination with PAD or MS detection is the method of choice for the analysis of all classes of carbohydrates including glycans. A novel anion-exchange stationary phase SweetSep™ has been developed that allows for fast and high resolution separation.

The new column enables for (a) fast, high-resolution separation of carbohydrates, (b) analysis of carbohydrates over a high molecular weight range: from mono– to oligosaccharides, (c) analysis of complex carbohydrate mixtures in food (e.g., honey, lactose in lactose-free labeled products, fructooligosaccharides, etc.).

Different classes of antibodies, including IgG1, 2 and 4, are immediately reduced down to light and heavy chain without the need of enzymes, denaturing or reducing agents using inline EC-LCMS. The workflow can be used for IdeS digested mAbs and does not affect the quantitation of acid labile species such as sialylated N-glycans.

The AEX200 column demonstrate (a) fast separation of mono-, di- and trisaccharides in various food sample matrices, (b)sensitive quantification of carbohydrates down to femtomole levels, (c) high-resolution separation of high-molecular weight oligosaccharides with a degree of polymerization up DP of 60.

Separation of all 6 glycan monosaccharides of interest and 2-Deoxy-D-Glucose (glycosylation inhibitor) was achieved within 7 minutes using the new SweetSep™ AEX20 column under isocratic elution conditions. Furthermore, profiling of released N-glycans from glycoproteins without the need for sample derivatization is shown using HPAEC-MS.

With a new electrochemical cell, complete reduction of inter- and intra-disulfide bonds in mAbs is achieved. For cystine knots peptides, the sequence coverage was 100%.

A simple and sensitive method is presented for the analysis of glyphosate and its metabolite AMPA in drinking water, using the ALEXYS HPAEC-PAD analyzer.

The ALEXYS HPLC-ECD Analyzer was used for the assay of Streptomycin sulphate in commercial pharmaceutical preparations, according to the official USP monograph.

An easy method is described for the analysis of nitrate and nitrite (E249 - E252) in food products, using the ALEXYS HPLC-UV analyzer.

HPLC with electrochemical detection is the method of choice for water- and fat-soluble vitamin analysis including ubiquinols.

Thursday, November 4, 2021 at 11am EDT | 8am PDT | 3pm GMT | 4pm CET HPLC with state-of-the-art electrochemical detection, the most sensitive and affordable detection technique. Learn about the tremendous power of Electrochemical Detection!

The sensitive analysis of carbohydrates in instant coffee, based on an adapted ISO 11292:1995 method, is shown using the ALEXYS Carbohydrates Analyzer.

A fast and sensitive method is described for the analysis of phytosterols (sterols and stanols) using the ALEXYS HPLC-ECD analyzer equipped with a BDD WE electrode.

Impurity analysis of β-cyclodextrin in sulfobutyl ether β-cyclo-dextrin sodium according to the exact method described in the USP monograph.

Gentamicin sulphate analysis in pharmaceutical preparations according to the official USP (37-NF32) and EP monograph (8.0) using the ALEXYS HPLC-ECD analyzer.

H2O2 is often used as an oxidizing agent in toothpaste. A fast and sensitive method is presented to allow the quantitation of hydrogen peroxide in less than 3 min.

With a new electrochemical cell, complete reduction of inter- and intra-disulfide bonds in mAbs is achieved with superior sequence coverage.

The ALEXYS Carbohydrate Analyzer is a dedicated instrument for the routine analysis of sugars and oligosaccharides in F&B products based on HPAEC-PAD.

Erythromycin analysis in creams, lotions, etc., based on HPAEC-PAD using the ALEXYS Antibiotics Analyzer according to the official 2018 USP 41—NF36 monograph.

SMX and TMP are the active ingredients of Co-trimoxazole or Bactrim. They can be analyzed easily and inexpensively using HPLC with pulsed amperometric detection.

The ALEXYS HPAEC-PAD Analyzer is used for the analysis of both antibiotics, in accordance with the USP 38 monographs.

A HPAEC-PAD method is presented for the sensitive analysis of mono-, di-, and trisaccharides in plant tissue extracts and root exudates..

This application note demonstrates a fast, selective, and sensitive method for the analysis of lactose and isomers based on HPAEC with Pulsed Amperometric Detection.

A HPAEC-PAD method with a four-step potential waveform is applied for the analysis of Erythritol, Xylitol, Sorbitol, Mannitol, and Maltitol, resulting in excellent reproducibility.

The ALEXYS Antibiotics Analyzer is used for routine assay and impurity analysis of Azithromycin in various formulations according USP 41 monographs.

The analysis of mono-, di-, and more complex oligosaccharides is demonstrated using the ALEXYS Carbohydrates analyzer according to the AOAC official method 2019.

The ALEXYS Carbohydrates Analyzer is a robust and reliable solution for the analysis of carbohydrates in apple juice, buttermilk, malt beer, and artificial sweeteners.