Claudia Seidl | Authors


Column Re-Equilibration Following Gradient Elution: How Long is Long Enough? Part 1: Reversed-Phase and HILIC Separations of Small Molecules

For decades the prevailing perception was that satisfactory re-equilibration of reversed-phase columns following gradient elution took a long time. In the early 2000s we showed that this perception was not well founded, and demonstrated that adequate re‑equilibration could be achieved in seconds. Recently, we have shown the same for HILIC columns. All of this work so far has been with small molecules. In this article, we present an overview of this work, and summarize the practical utility of it all.