Davy Guillarme

This free software allows teachers to illustrate the basic principles of HPLC, such as chromatographic resolution, the van Deemter equation, and how to manipulate or adapt retention in reversed-phase HPLC.

The recent trends in column technology for reversed-phase LC, SEC, IEX, and HIC for analysis of biopharmaceuticals at the protein level is critically discussed.

Modern Column Technologies for the Analytical Characterization of Biopharmaceuticals in Various Liquid Chromatographic Modes

Experimental results and method validation data from two analytical case studies of complex small?molecule pharmaceuticals illustrates the utility and advantages of UHPLC in high-resolution separations.

Experimental results and method validation data from two case studies of complex small-molecule pharmaceuticals illustrate the utility and advantages of UHPLC for quality control.

This review provides an updated overview of the theory behind the success of SPP technology, trends, benefits, and limitations. It also summarizes the latest developments of sub-2-?m SPPs and instrumental constraints associated with their use.

An updated overview of the development of SPP technology, including benefits, limitations, and the theory behind its success

Hydrophilic interaction liquid chromatography (HILIC) has recently become more important, particularly for the analysis of polar drugs, metabolites and biologically relevant compounds in glycomics, proteomics, metabolomics and clinical analysis. HILIC makes it possible to increase the retention of polar compounds, achieve orthogonal selectivity and increase mass spectrometry (MS) sensitivity, compared with reversed-phase liquid chromatography. This article discusses the advantages and limitations of HILIC in a variety of practical applications in the pharmaceutical industry.

Two promising wide-pore stationary phases were recently introduced for the fast and high resolution separations of large biomoleculaes: the recently launched widepore 3.6?m superficially porous particle and the hybrid-type sub 2-?m fully porous particle. This article focuses on the achievable throughout and resolution for the characterization of biomolecules.

Two promising wide-pore stationary phases were recently introduced for the fast and high-resolution separations of large biomolecules: the recently launched wide pore 3.6 ?m superficially porous particle and the hybrid-type sub-2-?m fully porous particle. This article focuses on the achievable throughput and resolution for the characterization of biomolecules.

Very short columns filled with 1.9 µm particles were evaluated for the ultra-fast analysis of pharmaceutical formulations. Local anæsthetic, mydriatic and anti-hypertensive agents were chosen as analytes and a method was developed and validated for each of these substances, according to ICH guidelines. Excellent quantitative performance was obtained using an optimized chromatographic system that reduces the importance of extra-column effects and cuts the analysis time to less than 15 s.

Many HPLC analyses could be performed at lower expenditure. This could involve a combination of reducing the analysis time, reducing the resolution between critical peaks, and lowering the consumption of mobile phase. Successfully optimizing the method in such instances - as well as in situations where it is necessary to transfer the method to another laboratory that lacks the same selection of columns - can save the analyst time and money.