Katarina Oden | Authors

The basic types of column overload (volume and mass) and the variables that most affect the sample loading capacity in PLOT column chromatography are examined.

Knowing the variables that most affect the sample loading capacity in a porous layer open tubular (PLOT) column is vital to determining how much sample to inject.

The effects of water on retention and selectivity in gas–solid chromatography of various adsorbent PLOT columns is reviewed.

Solid adsorbent gas chromatography (GC) columns, such as porous layer open tubular (PLOT) columns, are the best option for GC analysis of C1–C5 hydrocarbons, but water can affect retention and selectivity. We review the effects of water for different types of PLOT columns, and explain how to prevent or remediate the problem.