Takashi Kotsuka | Authors


Reversed-Phase HPLC Column at Extreme High Temperature (150°C or Higher)

In general, polymer-based columns have a broad pH range (pH 2 to 13), and some have high temperature tolerance (up to 150°C or higher). Considerably large selectivity changes can be obtained by varying analysis temperature and mobile phase pH. Having control on these two parameters over wide ranges can be especially useful in method development.