The Application Notebook-02-11-2011

The isolation of α-Methylstyrene from the crude reaction mixture seems easy. However the reaction mixture contains some dimethyl sulfoxide and the oily wetting agent from the sodiumhydride.

Phospholipids are important compounds for the formation of bilayer membranes in biological systems.

The demand for faster throughput in LC–MS drug discovery labs has increased throughout the years.

BioPharma Compass is a fully automated solution for the rapid characterization of biopharmaceutical products such as proteins, peptides, RNA, and DNA.

Application of core-shell technology to the USP assay for ibuprofen to reduce analysis time and increase sample throughput and productivity, while improving the sensitivity of the assay for the determination of ibuprofen related compound C, a known degradation product with adverse health effects.

A simple and efficient supported liquid extraction (SLE) procedure for the extraction of THC and metabolites from human plasma at low levels has been developed.

The method described employs a triple quadrupole mass spectrometer equipped with hyperbolic quadrupole rods for increased selectivity to prerform a trace level screening method for PCDD/Fs and PCBs and follows the well-established United States Environmental Protection Agency (US EPA) Method 1613A.

Tablet formulations of most major pharmaceutical drug products contain significant amounts of inactive ingredients (excipients) in their formulations.

The QuEChERS method of food sample preparation was used in conjunction with comprehensive two-dimensional gas chromatography time-of-flight mass spectrometry (GCxGC–TOFMS) to successfully identify PAH contaminants spiked in liquid commercial infant formula.

AGPC column and organic solvents are used to selectively pass large macro molecules such as lipids, resins, waxes, and other compounds from sample extracts while retaining the smaller target analyte molecules in the pores of the gel.

Recognition of DNAs and RNAs wide range of biological actions has driven modern biopharmaceutical research into the therapeutic value of oligonucleotides.

This rapid LC–MS method shows sensitive and selective separation of five ethanolamine analytes in wastewater.

The US Pharmacopeia recently released USP<467> as the current monograph for determining residual solvents in pharmaceutical products by static headspace.

An approach was developed to align release and end-expiry specifications for molecular size for the quality control of Merck's polyvalent pneumococcal polysaccharide vaccine (PNEUMOVAX 23).

Biodiesel fuels are part of a rapidly growing alternative fuel industry to replace gasoline with renewable, more efficient, and lower emission fuels (1).

Flexible synthetic fused silica capillary is routinely supplied to the market in long continuous lengths on low cost Styrofoam or plastic spools.

An analytical method for the rapid determination of 25-OH vitamin D2 and 25-OH vitamin D3 in serum, which will speed diagnosis of potential Vitamin D deficiencies, is described.

Organochlorine pesticides are man-made organic chemicals with a history of wide spread use in the United States, as well as globally.

Aminopolycarboxylate chelating agents form stable complexes with transition metals, thereby increasing metal solubility, preventing metal-catalyzed reactions, and removing heavy metal contamination in soil remediation.

Allantoin is a heterocyclic organic compound derived from purine.

Gelatin is the denatured form of collagen, the main component of skin, bone, and connective tissues of animals.

Clavulanic acid is commonly used in combination with penicillin and cephalosporin antibiotics to overcome bacterial resistance.

Sweeteners are low or zero-calorie sugar substitutes that are added in drinks to provide the sweet taste of sucrose or table sugar.

Many modern stationary phases suffer from poor chromatographic performance for basic compounds at low pH, especially when using low-ionic strength mobile phases such as formic acid.

Natural gas and other gaseous fuels contain varying amounts and types of sulfur compounds which can be corrosive to equipment and can inhibit or destroy gas processing catalysts.

This application note demonstrates the extraction of sour cream, cream cheese, coffee creamer, heavy whipping cream, and low fat milk using an ASE 350 Accelerated Solvent Extractor technique developed by Dionex and compares results to the traditional Mojonnier technique.

Saccharides are fundamental substances that express various bioactivities and may exist independently or form complexes with proteins or lipids.

A modified QuEChERS-type extraction method is used with an additional concentration step to detect 38 anthelmintic (nematicides, flukicides, endectocides) drug residues, consisting of benzimidazoles, avermectins, and flukicides in milk. UHPLC–MS-MS instrumentation detects most analytes at <1 µg/kg.

Nicotine is a famously addictive alkaloid found in tobacco. Controlled doses of nicotine compounds are sometimes used to help tobacco users quit smoking.

This February 2011 edition of The Application Notebook is the 10th Anniversary of publishing these supplements and further affirms the valuable service they perform in the chromatography community.