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The Application Notebook-03-01-2009

The Application Notebook

Melamine and Cyanuric Acid Detection in 5 Minutes using LC–MS

March 02, 2009

Food and Beverage

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Since September 2008, 294 000 infants and young children suffered urinary problems as a result of the contamination of melamine in infant milk powder and were hospitalized. This hospitalization was required to treat the symptoms caused by the ingestion of melamine contaminated infant formula and related dairy products. Previously in 2007, pet food, animal feed wheat gluten and other protein-based foods were found to contain residues of melamine and its degradation product cyanuric acid.

Application of Novel Ethylene Bridged Hybrid Particles for Hydrophilic-Interaction Chromatography

March 02, 2009

General

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Using HILIC with highly efficient ethylene bridged hybrid (BEH) particles results in faster methods that exhibit improved polar retention, higher sensitivity, enhanced chromatographic resolution and significantly improved column lifetime.

The Integration of Microextraction Packed Sorbent (MEPS) into Multidimensional Strategies

March 02, 2009

General

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LC/GC approaches to analysis are attractive because they combine the selectivity of solid-phase sorbents in the first dimension with the separating power and peak capacity of capillary GC in the following dimensions.

Alternate Selectivity for Polar Compounds in Hydrophilic Interaction Liquid Chromatography (HILIC) Using a New Amino Type HILIC Column

March 02, 2009

General

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Hydrophilic interaction liquid chromatography (HILIC) offers unique advantages for the separation of very polar compounds when compared to reversed-phase chromatography. A new silica based HILIC phase was developed to provide additional selectivity options in HILIC separations. The separation of water soluble vitamins on the new TSKgel NH2-100 HILIC column and on the well known TSKgel Amide-80 HILIC column demonstrates the differences in selectivity.

Identification of Psychotropic Substances in Mushrooms by UHPLC–MS

March 02, 2009

Medical/Biological

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Forensic laboratories face a daunting task to identify trace amounts of controlled substances in small samples of seized evidence. Unambiguous identification is required to meet the stiff challenge that is sure to be raised in the courtroom. Positive proof is especially difficult to establish if the controlled substance is hidden in a complex food matrix with a high content of sugars, fats, fatty acids, proteins and alkaloids.

Trace Level Analysis of Melamine in Milk Products on 7890A/5975C Using a New Agilent J&W DB-5ms Ultra Inert Column and SamliQ SCX Cartridges

March 02, 2009

Food and Beverage

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GC–MS method is presented for the quantitative determination and confirmation of melamine residues in milk products. The milk sample was cleaned up using Agilent's SampliQ SCX SPE cartridges before derivatization. The derived extracts were analysed by GC–MS with EI in synchronous SIM/scan mode on a Agilent J&W DB-5ms Ultra Inert column.

Determination of Penicillins in Meat by High Performance Liquid Chromatography (HPLC-UV) and HPLC–MS–MS

March 02, 2009

Food and Beverage

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Penicillins are regulated substances in food for human consumption. The minimum residue limits range from 1 ng/g to as high as 50 ng/g depending on the compound. Because of the complexity of food matrices measuring the level of contaminants requires a total solution composed of sample extraction, sample clean-up, chromatographic separation and detection. In this paper, a solid-phase extraction method with a high performance liquid chromatography tandem mass spectrometry method (HPLC–MS–MS) is shown for the simultaneous determination of six antibiotic residues.