Eric S. Grumbach | Authors

Waters Corporation


Development and Validation of a UHPLC Method for Paroxetine Hydrochloride

An ultrahigh-pressure liquid chromatography (UHPLC) method was developed to separate paroxetine from several of its related compounds using a systematic screening protocol that monitors combinations of selectivity factors including column chemistry, organic modifier, and pH. When the best combination of these factors was selected, the method was optimized by varying gradient slope and temperature.