Eric S. Grumbach

Using HILIC with highly efficient ethylene bridged hybrid (BEH) particles results in faster methods that exhibit improved polar retention, higher sensitivity, enhanced chromatographic resolution and significantly improved column lifetime.

Using HILIC with highly efficient ethylene bridged hybrid (BEH) particles results in faster methods that exhibit improved polar retention, higher sensitivity, enhanced chromatographic resolution, and significantly improved column lifetime.

An ultrahigh-pressure liquid chromatography (UHPLC) method was developed to separate paroxetine from several of its related compounds using a systematic screening protocol that monitors combinations of selectivity factors including column chemistry, organic modifier, and pH. When the best combination of these factors was selected, the method was optimized by varying gradient slope and temperature.

Eric S. Grumbach, Diane M. Diehl and Jeffrey R. Mazzeo, Waters Corporation

The use of a silica column for HILIC is examined, including mechanistic studies of analyte retention on silica in aqueous-organic mobile phases.

This article describes a method development approach that uses different packing materials and mobile-phase pH levels to optimize the chromatographic separation of degradation products from active ingredients.