The Column-01-15-2013

In biopharmaceutical development the stability of biological molecules in drug formulations is important. This article describes three different approaches to the measurement and quantification of aggregates in protein solutions, and highlights the range of information that each technique can provide about the sample.

A new strain of the enzyme ß-glucosidase isolated from the microbial strain Aureobasidium pullulans (Sp? ß-gl) improves the aroma of wine, according to a team of Brazilian researchers.1

There has been a significant resurgence in the development of antibody-drug conjugates (ADC) as target-specific therapeutic agents for cancer treatments. This article presents a method for DAR determination based on size-exclusion chromatography with multi-angle light scattering (SEC?MALS) combined with ultraviolet (UV) absorption and differential refractive index (dRI) detection.

Would the truly quantitative detector please stand up!

Previously unreported biological activity of endocrine disrupting chemicals (EDCs) has been detected in water samples from 14 states in the US by researchers at the National Cancer Institute, Maryland, USA.1

"Lean" approaches are used to reduce wastes and costs when performing chromatographic procedures. The terminology of this approach is introduced in this article, together with illustrative examples and suggestions.