Characterization of Antibody-Drug Conjugates by SEC with Combined Light Scattering, dRI and UV Detection

January 15, 2013

The Column

The Column, The Column-01-15-2013, Volume 9, Issue 1

There has been a significant resurgence in the development of antibody-drug conjugates (ADC) as target-specific therapeutic agents for cancer treatments. This article presents a method for DAR determination based on size-exclusion chromatography with multi-angle light scattering (SEC?MALS) combined with ultraviolet (UV) absorption and differential refractive index (dRI) detection.

There has been a significant resurgence in the development of antibody‑drug conjugates (ADC) as target-specific therapeutic agents for cancer treatments. A critical factor for ADC formulations is the drug‑to‑antibody ratio (DAR), which describes the degree of modification. This article presents a method for DAR determination based on size-exclusion chromatography with multi-angle light scattering (SEC–MALS) combined with ultraviolet (UV) absorption and differential refractive index (dRI) detection.

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