LCGC Asia Pacific-06-07-2016
Generic UHPLC Method for the Simultaneous Analysis of Compounds with a Wide Range of Polarities
June 7th 2016Appropriate analytical methods are required to evaluate the presence, metabolism, degradation, and removal of specific compounds in complex mixtures. There is an increasing demand to analyze samples with a wide range of polarities in a variety of applications, including environmental analysis, biomarker discovery, and proteomics. Multiple analyses on complementary columns are often needed to cover the separation of all compounds with a large difference in polarity. This article describes a generic method involving an ultrahigh‑pressure liquid chromatography (UHPLC) system equipped with two external switching valves to connect hydrophilic interaction liquid chromatography (HILIC) and reversed-phase LC columns in series for the sequential analysis of polar and apolar compounds.
Nontargeted Metabolite Profiling in Next-Generation Plant Breeding: A Case Study in Malting Barley
June 7th 2016Nontargeted metabolite profiling by ultrahigh-pressure liquid chromatography–mass spectrometry (UHPLC–MS) is a powerful technique to investigate the influence of genetic and environmental factors on metabolic phenotypes in plants. The approach offers an unbiased and in-depth analysis that can reveal molecular markers of desirable phenotypic traits that can be complementary to genetic markers in plant breeding efforts. Here, the power of nontargeted metabolite profiling is illustrated in a study focused on the determination of molecular markers in malting barley that are predictive of desirable malting quality for brewing applications.
