D. Cabooter | Authors

HTC-17 will be held in person from 18 to 20 May 2022, at The Aula in Ghent, Belgium. Here’s a taste of what to expect.

HTC-17 will be held in person from 26 to 28 January 2022, at Conference Center Het Pand in Ghent, Belgium. Here’s a taste of what to expect.

The 17th International Symposium on Hyphenated Techniques in Chromatography and Separation Technology (HTC-17) will be held in person from 26 to 28 January 2022, at Conference Center Het Pand in Ghent, Belgium. HTC-17 will be organized under the auspices of the Royal Flemish Chemical Society (KVCV) and the Separation Science Group of the Royal Society of Chemistry (SSG RSC).

The main objective of this review article is to provide a clear summary of the different methods that can be used to quantify endogenous small molecules. Because of the increased use of mass spectrometry (MS) in the field of bioanalysis, a special focus will be placed on quantification by liquid chromatography (LC)–MS. Practical recommendations to face this bioanalytical challenge, in particular in terms of method validation, will also be provided.

The main objective of this review article is to provide a clear summary of the different methods that can be used to quantify endogenous small molecules. Because of the increased use of mass spectrometry (MS) in the field of bioanalysis, a special focus will be placed on quantification by liquid chromatography (LC)–MS. Practical recommendations to face this bioanalytical challenge, in particular in terms of method validation, will also be provided.

The packed particle bed format still rules LC columns, but advances continue in monoliths. Meanwhile, newer formats are on the horizon, including microfabricated columns and 3D printed columns. This article provides a critical review of all these technologies and demonstrates how further development of chromatographic columns will be of paramount importance in the future.