LCGC Europe-01-01-2006

The effects of increased data demand coupled with the torrential data outflow of our instruments can overwhelm even the most IT-savvy.

Guest columnist Matt Przybyciel reviews the structure, behaviour and applications of both alkyl- and phenyl-fluorinated phases in HPLC. The author presents some guidelines on potential areas of application for alkyl and phenyl-fluorinated phases, in which conventional C8, C18 and phenyl phases might have insufficient selectivity or too strong retention.

Improvement of detection and quantification limits for LC methods is a result of taking advantage of all the tools you have at your disposal.

An important prerequisite of a good sampling procedure for skin sebum is its reproducibility.

This article presents an overview of the work performed to define generic separation strategies and methods in chiral method development using capillary electrochromatography as a separation technique. Polysaccharide chiral stationary phases were found suitable for this purpose. Two separate strategies were defined, one for acidic and one for non-acidic substances. These strategies were evaluated and found applicable on structurally diverse molecules, showing their generic character.