LCGC Europe-01-01-2006

LCGC Europe

Fluorinated HPLC Phases — Looking Beyond C18 for Reversed-Phase HPLC

January 01, 2006

Column Watch

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Guest columnist Matt Przybyciel reviews the structure, behaviour and applications of both alkyl- and phenyl-fluorinated phases in HPLC. The author presents some guidelines on potential areas of application for alkyl and phenyl-fluorinated phases, in which conventional C8, C18 and phenyl phases might have insufficient selectivity or too strong retention.

Generic Capillary Electrochromatographic Screening and Optimization Strategies for Chiral Method Development

January 01, 2006

Capillary Electrochromatography

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This article presents an overview of the work performed to define generic separation strategies and methods in chiral method development using capillary electrochromatography as a separation technique. Polysaccharide chiral stationary phases were found suitable for this purpose. Two separate strategies were defined, one for acidic and one for non-acidic substances. These strategies were evaluated and found applicable on structurally diverse molecules, showing their generic character.