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LCGC Europe-04-01-2007

LCGC Europe

Buffer Preparation — Hints, Tips and Common Errors

April 01, 2007

CE Currents

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4

Accurate preparation and correct selection of buffers is essential to obtain reproducible and consistent results in capillary electrophoresis (CE). A number of factors should be considered in buffer optimization, including the pKa of the buffering ion and the analyte as well as the molarity of the acid or base used in the adjusting procedure. Accurate recording of the precise reagents used and the procedures performed is necessary to prepare buffers consistently. "Poor reproducibility of results and poor quantitative precision will be attainable in CE assays without significant attention being paid to the preparation of buffers used."

New Technologies

April 01, 2007

New Technologies

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With a frequency response of 90 Hz–20 kHz, these LaCie FireWire speakers deliver powerful, vibrant sound for your music, movies and games. The speakers connect easily to computers, either laptops or desktops, as well as MP3 players, using the built-in FireWire cable. FireWire provides superior digital audio as well as line power - no need for batteries or an AC adapter.

LC Workshops

April 01, 2007

Event News

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Three LC workshops will take place between 30 April and 4 May at the Chesterfield Mayfair Hotel, London, UK. The courses offer practical training aimed at improving chromatography skills to enhance laboratory performance. They are hosted by LCGC Europe in association with LC Resources and sponsored by Hichrom.

Pressure-driven Chromatography in Perfectly Ordered Pillar Array Columns

April 01, 2007

Pressure-driven Chromatography

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4

his article reveals the first liquid chromatography (LC) separations performed on a microfabricated pillar array column under pressure-driven conditions. The pillars were non-porous and produced using a Bosch-type deep reactive ion etch (DRIE) to pattern the surface of a silicon wafer and had a diameter of approximately 5 μm. Two different packing densities were compared: one similar to the packing density of a packed bed (external porosity of approximately 49%) and one similar to the packing density of monolithic columns (external porosity of approximately 70%).