LCGC Europe-04-01-2007

Accurate preparation and correct selection of buffers is essential to obtain reproducible and consistent results in capillary electrophoresis (CE). A number of factors should be considered in buffer optimization, including the pKa of the buffering ion and the analyte as well as the molarity of the acid or base used in the adjusting procedure. Accurate recording of the precise reagents used and the procedures performed is necessary to prepare buffers consistently. "Poor reproducibility of results and poor quantitative precision will be attainable in CE assays without significant attention being paid to the preparation of buffers used."

Because GPC is a reliable, simple and fast characterization method it can be readily adopted to investigate the separation behaviour of membranes.

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Detector selection for a method should be made on a case-by-case basis.

The complexity of the systems that are used vary from standard GC configurations to assess the effectiveness of protective clothing to trace analysis of biomedical samples with complex GC configuration.

Three LC workshops will take place between 30 April and 4 May at the Chesterfield Mayfair Hotel, London, UK. The courses offer practical training aimed at improving chromatography skills to enhance laboratory performance. They are hosted by LCGC Europe in association with LC Resources and sponsored by Hichrom.

his article reveals the first liquid chromatography (LC) separations performed on a microfabricated pillar array column under pressure-driven conditions. The pillars were non-porous and produced using a Bosch-type deep reactive ion etch (DRIE) to pattern the surface of a silicon wafer and had a diameter of approximately 5 μm. Two different packing densities were compared: one similar to the packing density of a packed bed (external porosity of approximately 49%) and one similar to the packing density of monolithic columns (external porosity of approximately 70%).