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LCGC North America-02-01-2019

LCGC North America

GCxGC: From Research to Routine

February 01, 2019

Column: GC Connections

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Two-dimensional gas chromatography (GCxGC) is becoming the technique of choice for analysis of highly complex samples such as petroleum, pharmaceuticals, biological materials, food, flavors, and fragrances. Here, we explain how GCxGC works and provide examples that illustrate its advantages.

Reversed-Phase Liquid Chromatography and Water, Part I: How Much is Too Much?

February 01, 2019

LC Troubleshooting

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In reversed-phase separations, retention generally increases as the fraction of water in the eluent increases. When we encounter situations where retention is too low for an analyte of interest, we tend to use eluents with higher and higher levels of water. But how much water is too much?

Vol 37 No 2 LCGC North America February 2019 Regular Issue PDF

February 01, 2019

LCGC February 2019 Issue PDF

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Click the title above to open the LCGC North America February 2019 regular issue in an interactive PDF format.

Data Integrity Focus, Part II: Using Data Process Mapping to Identify Integrity Gaps

February 01, 2019

Featured Articles

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Data integrity is paramount when working in a regulated environment. Data process mapping is an excellent way to identify and mitigate data gaps and record vulnerabilities in a chromatographic process. This approach is simple and practical.

Optimizing Sensitivity in GC–FID Analysis

February 01, 2019

The Essentials

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It is often possible to achieve better sensitivity and lower limits of detection and quantitation using standard gas chromatography instrumentation. Paying attention to fundamental variables-like sample solvent, initial oven temperature hold, carrier gas operating mode, and thermal gradients- can lead to much improved method performance.

Separation of Five Steroids on a Hamilton PRP-C18 Reversed-Phase HPLC Column

February 01, 2019

Medical/Biological

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In this study, a reversed-phase HPLC method was developed for separation of five steroid hormones with partition coefficients ranging from 1.47 (cortisone) to 4.5 (pregnenolone) on a Hamilton PRP-C18 HPLC column.

Characterization of TSKgel FcR-IIIA-NPR HPLC Column by Top Down Mass Spectrometry

February 01, 2019

Medical/Biological

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The purpose of this study is to demonstrate the use of mass spectrometry to characterize the elution profile of a typical IgG1 molecule separation on a TSKgel FcR-IIIA-NPR column, and verify the observations that certain glycan structures impart higher activity to the monoclonal antibody, especially as it relates to the presence of terminal galactose sugars.