LCGC North America-02-01-2019

LCGC North America
Column: GC Connections

February 01, 2019

Two-dimensional gas chromatography (GCxGC) is becoming the technique of choice for analysis of highly complex samples such as petroleum, pharmaceuticals, biological materials, food, flavors, and fragrances. Here, we explain how GCxGC works and provide examples that illustrate its advantages.

LCGC North America

In reversed-phase separations, retention generally increases as the fraction of water in the eluent increases. When we encounter situations where retention is too low for an analyte of interest, we tend to use eluents with higher and higher levels of water. But how much water is too much?

LCGC February 2019 Issue PDF
LCGC North America

February 01, 2019

Click the title above to open the LCGC North America February 2019 regular issue in an interactive PDF format.

Data integrity is paramount when working in a regulated environment. Data process mapping is an excellent way to identify and mitigate data gaps and record vulnerabilities in a chromatographic process. This approach is simple and practical.

LCGC North America
Featured Articles

February 01, 2019

The contributions of Milos V. Novotny and Ken Broeckhoven, the winners of the 2019 LCGC Lifetime Achievement and Emerging Leader in Chromatography Awards, are presented.

LCGC North America
The Essentials

February 01, 2019

It is often possible to achieve better sensitivity and lower limits of detection and quantitation using standard gas chromatography instrumentation. Paying attention to fundamental variables-like sample solvent, initial oven temperature hold, carrier gas operating mode, and thermal gradients- can lead to much improved method performance.

This applicate note presents the use of an automated solid-phase extraction (SPE) system to improve the accuracy and precision of semivolatile extractions, in compliance with EPA Method 8270.

One of the fundamental tenets of chromatography is the interaction between an analyte and stationary phase.

LCGC North America

In this study, a reversed-phase HPLC method was developed for separation of five steroid hormones with partition coefficients ranging from 1.47 (cortisone) to 4.5 (pregnenolone) on a Hamilton PRP-C18 HPLC column.

LCGC North America

This method showed excellent precision and accuracy for the 12 FDA regulated mycotoxins that were evaluated during a validation study that covered a variety of matrices.

LCGC North America

Pickering Laboratories developed an analytical method to comply with all the chromatographic requirements of Commission Regulation (EC) No 152-2009.

LCGC North America
Pharmaceutical/Drug Discovery

February 01, 2019

For your highly sensitive UHPLC–MS analyses, how can you reduce noise and additional signals to a minimum?

LCGC North America
Medical/Biological

February 01, 2019

Chitosans are analyzed by SEC-MALS to determine molar mass moments and distributions.

This application note presents calibration plots, RSDs, and MDLs for IEC Method 62321-8 using a CDS Model 6150.

LCGC North America

The purpose of this study is to demonstrate the use of mass spectrometry to characterize the elution profile of a typical IgG1 molecule separation on a TSKgel FcR-IIIA-NPR column, and verify the observations that certain glycan structures impart higher activity to the monoclonal antibody, especially as it relates to the presence of terminal galactose sugars.

This application note effectively outlines a simple three-step method for the extraction of loperamide and its main metabolite, N-desmethyl loperamide, from blood.

LCGC North America
Pharmaceutical/Drug Discovery

February 01, 2019

Here we applied our newly developed UHPLC C18 and C30 columns to oligonucleotide analysis.

In this application, a simple method to simultaneously analyze various water-soluble vitamins was developed.