LCGC North America-12-01-2004

The authors look at two types of method modification from a scientific and regulatory standpoint: a change of column and a change in the operating system. Both are difficult to avoid if a method is used for several years.

While some variation in retention is normal, larger changes from run to run are generally indicative of a problem. Here, the auther discusses how the retention time of a peak in an LC separation can be a useful diagnostic tool to identify problems with a separation.

In this first of a two-part installment, John Hinshaw discusses the relative effects that inlet pressures and oven temperatures have on capillary gas chromatography.