LCGC North America-12-01-2004

LCGC North America

Adjusting Conditions for a Routine Reversed-Phase HPLC Assay, Part I: Changing the Column

December 01, 2004

Column Watch

22

12

The authors look at two types of method modification from a scientific and regulatory standpoint: a change of column and a change in the operating system. Both are difficult to avoid if a method is used for several years.

2004 Index of Authors and Subjects

December 01, 2004

Index

22

12

Retention Time Changes

December 01, 2004

LC Troubleshooting

22

12

While some variation in retention is normal, larger changes from run to run are generally indicative of a problem. Here, the auther discusses how the retention time of a peak in an LC separation can be a useful diagnostic tool to identify problems with a separation.

Peaks of Interest

December 01, 2004

22

12

December 2004 TOC

December 01, 2004

22

12

Flow, Pressure, and Temperature Calibration: Part I

December 01, 2004

GC Connections

22

12

In this first of a two-part installment, John Hinshaw discusses the relative effects that inlet pressures and oven temperatures have on capillary gas chromatography.