Adjusting Conditions for a Routine Reversed-Phase HPLC Assay, Part I: Changing the Column

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LCGC North America

LCGC North America, LCGC North America-12-01-2004, Volume 22, Issue 12

Columns | <b>Column: Column Watch</b>

The authors look at two types of method modification from a scientific and regulatory standpoint: a change of column and a change in the operating system. Both are difficult to avoid if a method is used for several years.