30-s UHPLC Separation of Pharmaceutical Ingredients using PDA Detection

March 2, 2010
Andre J. Nohl

,
Yenny Chan

,
Guifeng Jiang

The Application Notebook

Volume 0, Issue 0
Page Number: 14

Ultra fast separations of acetaminophen, caffeine, propofol and parabens are achieved in 30 s using the Thermo Scientific Accela UHPLC system with the 80 Hz PDA detector. The performance of the Accela PDA detector is highlighted by its low noise level, low drift, four orders of magnitude linear working range and high sensitivity.

Guifeng Jiang, Yenny Chan and Andre J. Nohl, Thermo Fisher Scientific Inc., San Jose, California, USA.

Ultra fast separations of acetaminophen, caffeine, propofol and parabens are achieved in 30 s using the Thermo Scientific Accela UHPLC system with the 80 Hz PDA detector. The performance of the Accela PDA detector is highlighted by its low noise level, low drift, four orders of magnitude linear working range and high sensitivity.

Results

Detector noise and drift

The drift was measured at flow rate of 1000 μL/min with a constant flow of 100% methanol. The drift value for the PDA detector was 0.1 mAU/h.

The reduction in the level of noise increased detector sensitivity. The level of noise of the PDA detector was lower when the detector was operated at lower data sampling rates and/or higher rise times. The levels of noise of four different data rates (5, 20, 40, 80 Hz) were 1.1, 4.5, 14.8 and 43.1 μAU respectively.

Data sampling rate optimization

In general, about 20 data points per peak is required for reproducible quantification. High data sampling rates resulted in increased noise reducing sensitivity. Eight pharmaceutical small molecules were separated in 30 s using LC gradient method I and a BDS 50 × 4.6 mm column with elution order of uracil, acetaminophen, caffeine, methylparaben, ethylparaben, propylparaben, butylparaben and propofol (Figure 1). Similar peak width and peak resolution were obtained with 40 Hz and 80 Hz data sampling rates, indicating that both rates were suitable for this chromatographic separation. The 40 Hz data sampling rate was preferred here for its lower noise level and better sensitivity.

Figure 1

Detector linearity and sensitivity

High throughput UHPLC completes separations in tens of seconds and improves sensitivity resulting in sharper, taller and better resolved peaks.

Calibration curves of the four compounds were constructed over the concentration range of 39–300000 ng/mL with 11 calibration levels. Correlation coefficients were achieved 0.999 or better for all four compounds with four orders of magnitude linearity working range.

Conclusions

The Accela PDA detector acquires data at sampling rates up to 80 Hz for UHPLC high throughput separations with independent setting of diode rates, data sampling rates and rise time. Using the current UHPLC instrumentation and sub-2 μm particle columns, the PDA detector provided sufficient data point to define a chromatographic peak with peak width as narrow as 0.166 s. The four orders of magnitude linear working range and the ppb (ng/mL) level sensitivity for the PDA detector ensured the low level determination and quantification of the target compounds in UHPLC high throughput analyses.

Accela is a trademark of Thermo Fisher Scientific Inc.

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