70-min Amino Acid Analysis of Physiological Samples
Pickering Laboratories specializes in the manufacturing of cation-exchange columns and eluants for amino acid analysis.
Pickering Laboratories specializes in the manufacturing of cation-exchange columns and eluants for amino acid analysis. Post-column derivatization with Ninhydrin offers unmatched selectivity and reproducibility of the analysis for most challenging matrixes.
Figure 1
Pinnacle PCX post-column derivatization system allows you to combine eluant gradient capabilities of modern HPLC instruments with column temperature gradients. We introduce accelerated amino analysis method for physiological samples that utilizes temperature and eluant gradients.
Table I: HPLC program
Table II: Column oven program
Pickering Laboratories, Inc
1280 Space Park Way, Mountain View, CA 94043
tel. (800)654-3330, (408)694-6700
Website: www.pickeringlabs.com
SEC-MALS of Antibody Therapeutics—A Robust Method for In-Depth Sample Characterization
June 1st 2022Monoclonal antibodies (mAbs) are effective therapeutics for cancers, auto-immune diseases, viral infections, and other diseases. Recent developments in antibody therapeutics aim to add more specific binding regions (bi- and multi-specificity) to increase their effectiveness and/or to downsize the molecule to the specific binding regions (for example, scFv or Fab fragment) to achieve better penetration of the tissue. As the molecule gets more complex, the possible high and low molecular weight (H/LMW) impurities become more complex, too. In order to accurately analyze the various species, more advanced detection than ultraviolet (UV) is required to characterize a mAb sample.
