Ultrahigh-pressure liquid chromatography–quadrupole time-of-flight mass spectrometry (UHPLC–QTOF MS) shows diet shapes honeybee metabolic responses to heat.
|Articles|August 2, 2012
- The Column-08-02-2012
- Volume 8
- Issue 14
Asymmetric Flow Field Flow Fractionation Coupled with ICP-MS for Speciation Analysis of Plasma Proteins
This work demonstrates the potential of the simultaneous on-line hyphenation of asymmetric flow field flow fractionation (AF4) to inductively coupled plasma-mass spectrometry (ICP-MS) and ultra violet-visible/multi angle light scattering (UV-vis/MALS) detection as a promising and simple tool to obtain information on the multi-element speciation and absolute molecular mass of human plasma proteins such as albumin and transferrin: the two main proteins that have been proposed to bind and transport metal ions in this biological fluid.
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This work demonstrates the potential of the simultaneous on-line hyphenation of asymmetric flow field flow fractionation (AF4) to inductively coupled plasma-mass spectrometry (ICP-MS) and ultra violet-visible/multi angle light scattering (UV-vis/MALS) detection as a promising and simple tool to obtain information on the multi-element speciation and absolute molecular mass of human plasma proteins such as albumin and transferrin.
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