Size exclusion chromatography (SEC) plus precipitation improves extracellular vesicle (EV) purity from blood plasma.
|Articles|August 2, 2012
- The Column-08-02-2012
- Volume 8
- Issue 14
Asymmetric Flow Field Flow Fractionation Coupled with ICP-MS for Speciation Analysis of Plasma Proteins
This work demonstrates the potential of the simultaneous on-line hyphenation of asymmetric flow field flow fractionation (AF4) to inductively coupled plasma-mass spectrometry (ICP-MS) and ultra violet-visible/multi angle light scattering (UV-vis/MALS) detection as a promising and simple tool to obtain information on the multi-element speciation and absolute molecular mass of human plasma proteins such as albumin and transferrin: the two main proteins that have been proposed to bind and transport metal ions in this biological fluid.
Advertisement
This work demonstrates the potential of the simultaneous on-line hyphenation of asymmetric flow field flow fractionation (AF4) to inductively coupled plasma-mass spectrometry (ICP-MS) and ultra violet-visible/multi angle light scattering (UV-vis/MALS) detection as a promising and simple tool to obtain information on the multi-element speciation and absolute molecular mass of human plasma proteins such as albumin and transferrin.
Articles in this issue
about 14 years ago
Nobel Laureate at Pittconabout 14 years ago
Breath analysisabout 14 years ago
Agilent acquisitionabout 14 years ago
Fine wine?about 14 years ago
Olympic testingabout 14 years ago
Tips & Tricks GPC/SEC: Faster Resultsabout 14 years ago
Market Profile: Continuous Flow Analysis and Discrete AnalysersAdvertisement
Related to this article
Nitish Sharma and Piotr Alvarez compare reverse-phase separations of small molecules vs. peptides, from pI value and hydrophobicity to impurity profiles.
Meet the expert panel exploring the challenges involved in GLP-1 analysis in this LCGC International Peer Exchange®.
Ultrahigh-performance liquid chromatography–tandem mass spectrometry (UHPLC–MS/MS) shows flonicamid residues in rice pose negligible dietary risk.
High-performance liquid chromatography-tandem mass spectroscopy (HPLC–MS/MS) chromatography reveals pyrrolizidine alkaloids (PA)/pyrrolizidine alkaloid N-oxides (PANO) toxin risks in 63 tea samples.
Advances in DNA/RNA chromatography columns led to stable, pure mRNA production, supporting the development of COVID-19 vaccines rapidly.
Steven Ray Wilson's AFFL LC–MS system runs thousands of injections per column, but he calls validation and standardization the field's real barrier.
Kinjal Bhatt and Ina Varfaj describe imposter syndrome fading through repeated exposure and conference talk rather than a single breakthrough moment.
Advertisement
Advertisement