A research group from AlbaNova University Center, Royal Institute of Technology (Stockholm, Sweden) developed an automated method for purifying and desalting proteins before mass spectrometry analysis.
A research group from AlbaNova University Center, Royal Institute of Technology (Stockholm, Sweden) developed an automated method for purifying and desalting proteins before mass spectrometry analysis. The automated method was able to prepare as many as 48 samples in a 4.5-h period and used only small volumes of buffer. The method enabled the analysis of 19,000–35,000 Da recombinant proteins from crude and clarified cell lysates by electrospray ionization and matrix-assisted laser desorption–ionization mass spectrometry and gel electrophoresis.
Dispersive Liquid-Liquid Microextraction: A Review and Roundup of Green Sample Prep Advancements
May 15th 2024The still relatively new technique has distinct advantages, but a few of those benefits make it incompatible with some of the currently accepted principles of green sample preparation.
Reflecting on the Influence of the Current State of Sample Preparation on GC, Part 2: Techniques
May 9th 2024In this installment, we examine trends in the use of sample preparation techniques through the lens of instrumental analysis by gas chromatography (GC) and GC–mass spectrometry (GC–MS).