Automating Sample Preparation for MS Analysis of Recombinant Proteins

June 1, 2009

A research group from AlbaNova University Center, Royal Institute of Technology (Stockholm, Sweden) developed an automated method for purifying and desalting proteins before mass spectrometry analysis.

A research group from AlbaNova University Center, Royal Institute of Technology (Stockholm, Sweden) developed an automated method for purifying and desalting proteins before mass spectrometry analysis. The automated method was able to prepare as many as 48 samples in a 4.5-h period and used only small volumes of buffer. The method enabled the analysis of 19,000–35,000 Da recombinant proteins from crude and clarified cell lysates by electrospray ionization and matrix-assisted laser desorption–ionization mass spectrometry and gel electrophoresis.