News|Articles|September 3, 2025

Best Practices for Pipetting 3D Cell Culture Hydrogels

Author(s)Sartorius

Discover best practices for reproducible 3D cell culture workflows. Learn how Picus® 2 pipettes and GrowDex® hydrogels improve accuracy, reduce variability, and streamline sample prep.


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Figure 2. Split injection used to measure triplicate test of MTBE, hexane, o-xylene, and 1-methylnaphthalene comparing different solvents (methanol, methylene chloride) and column insertion distances starting with 0.5 mm, 5.0 mm, 10.0 mm, 15 mm, and 20.0 mm. Methanol had the best performance at the 5.0 mm insertion distance and methylene chloride looked slightly better at the 0.5 mm. We would still recommend not going below the 5.0 mm manufacturer recommended insertion distance.
Revisiting insertion distance for methanol and methylene chloride in GC analysis and confirming that insertion depth strongly affects response and reproducibility.
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