Chiral Derivatizing Agents Aid Enantioseparation of Amino Acids

August 1, 2008

Researchers at the Indian Institute of Technology Roorkee (Roorkee, India) prepared chiral dichloro- and monochloro s-triazines by nucleophilic displacement of chlorine atoms in s-triazine chloride and its 6-methoxy derivative with various amino acid amides.

Researchers at the Indian Institute of Technology Roorkee (Roorkee, India) prepared chiral dichloro- and monochloro s-triazines by nucleophilic displacement of chlorine atoms in s-triazine chloride and its 6-methoxy derivative with various amino acid amides. The diastereomers were then separated using reversed-phase liquid chromatography with a C18 column and an acetonitrile-aqueous trifluoroacetic acid mobile phase. The researchers found that the dichloro s-triazine reagents enabled better diastereomer separation than the monochloro s-triazines.