LCGC UST
|Articles|March 2, 2016

Comparison of SPE vs. SLE for the Quantitative Analysis of Anabolic Steroids in Serum Using LC-MS/MS

UCT’s approach for the analysis of anabolic steroids from serum utilizes a traditional reversed phase interaction, but also features the addition of strong-anion exchange functionality (QAX) within the sorbent. This added functionality aids in the removal of unwanted matrix components commonly found in serum such as amino acids and inorganic ions. To prove the effectiveness of this approach, a comparison study was conducted using UCT’s recommended sorbent for steroid analysis (C8 + QAX) versus a traditional SLE, diatomaceous earth sorbent.

 


Related to this article

Best of the Week: From Olive Waste to mRNA Vaccines
This week's Chromatography Online highlights include olive waste antioxidant recovery, food and tea analysis, EV isolation, mRNA vaccine purification, perfusion chromatography for oligonucleotides, mass spectrometry in teaching and career advice from “Women in Chromatography.”