Comparison of SPE vs. SLE for the Quantitative Analysis of Anabolic Steroids in Serum Using LC-MS/MS
UCT’s approach for the analysis of anabolic steroids from serum utilizes a traditional reversed phase interaction, but also features the addition of strong-anion exchange functionality (QAX) within the sorbent. This added functionality aids in the removal of unwanted matrix components commonly found in serum such as amino acids and inorganic ions. To prove the effectiveness of this approach, a comparison study was conducted using UCT’s recommended sorbent for steroid analysis (C8 + QAX) versus a traditional SLE, diatomaceous earth sorbent.
High pH and Temperature with HALO® ELEVATE C18, 1.5 mm ID Column
December 11th 2024Over the course of 5000 injections, the HALO®Elevate C18 1.5 mm ID column demonstrates stability and robustness in the stationary phase and the column hardware, with no significant changes to the retention time, peak shape, and back pressure.
Separation of Antidepressants Using HALO 90 Å PCS C18 Compared to C18
December 11th 2024A mix of four antidepressants is separated using the HALO®PCS (positive charged surface) C18 column demonstating the improved tailing factor and efficiency when compared to a traditional (uncharged) C18 stationary phase.
